J J Jiang，D W Chen，B Yu，J He，J Yu，X B Mao，Z Q Huang，Y H Luo，J Q Luo， P Zheng
本研究的目的是评价液体喂养对断奶早期仔猪生长性能、营养物质消化率和肠道屏障功能的影响。将360头24d平均体重为6.98±0.15 kg的断奶仔猪(杜洛克×长白×约克)随机分为两组，分别为：对照组（基础日粮干饲，CON组）和试验组（将基础日粮与水以1：4的比例混合湿饲，LF组)，每处理组6个重复，每重复30头，试验为期7d。在第4 ~ 7d，采集新鲜粪便样本，评价营养物质表观总消化道消化率（ATTD）。7d后在每个重复中选取2头断奶仔猪安乐死，取生理样品。
试验数据显示，与CON组相比，LF组：显著提高了猪只平均日增重（ADG，分别为281 g 和183 g）、平均日采食量（ADFI， 分别为374 g 和 245 g）和终体重（BW，分别为8.95 kg和8.26 kg)，P < 0.05；显著降低了血清皮质醇、乳酸浓度及二胺氧化酶活性，P < 0.05；提高了乙醚提取物和灰分的ATTD，P < 0.05；增加了空肠粘膜淀粉酶、脂肪酶和乳糖酶活性，P < 0.05。此外，LF组具有较高的绒毛高度和绒毛高度:隐窝深度比，P < 0.05，增加了空肠胰岛素样生长因子-1受体（IGF1-R）、紧密连接蛋白-2抗体 （CLDN-2）、闭锁连接蛋白-1 （ZO-1）和闭锁连接蛋白-2 （ZO-2）的 mRNA表达，P < 0.05。此外，LF组猪只盲肠内容物中总细菌和大肠杆菌的丰度较低，乙酸和丁酸浓度较高，P < 0.05。
Liquid feeding, a widely used technique, has been applied as a feeding technique commonly in global swine production. The objective of this study was to evaluate the effects of liquid feeding on growth performance, nutrient digestibility, and intestinal barrier functions during the early weaning period in pigs. Three hundred and sixty 24-d-old weanling pigs (Duroc × Landrace × Yorkshire) with BW of 6.98 ± 0.15 kg were randomly assigned to a control diet (dry fed basal diet, CON) or as meal mixed with water in the ratio 1:4 (liquid fed basal diet, LF) with 6 replicates per treatment and 30 weanling pigs per replicate. The study lasted 7 d. On days 4-7, fresh fecal samples were collected to evaluate apparent total tract digestibility (ATTD) of nutrients. After 7 d, 2 weanling pigs per pen were euthanized and physiological samples were obtained. Results showed that LF increased (P < 0.05) ADG (281 g vs. 183 g), ADFI (374 g vs. 245 g) and final BW (8.95 kg vs. 8.26 kg) compared to CON. Compared with CON, LF significantly decreased (P < 0.05) serum cortisol and D-lactate concentrations as well as the activity of diamine oxidase, enhanced (P < 0.05) the ATTD of ether extract and ash, increased (P < 0.05) the activities of amylase, lipase and lactase in the jejunal mucosa. Furthermore, LF had higher (P < 0.05) villus height and villi height:crypt depth, and increased (P < 0.05) mRNA expressions of insulin-like growth factors-1 receptor (IGF1-R), claudin-2 (CLDN-2), zonula occludens-1 (ZO-1) and zonula occludens-2 (ZO-2) in the jejunum. Moreover, LF had lower (P < 0.05) abundances of total bacteria and Escherichia coli and higher (P < 0.05) concentrations of acetic acid and butyric acid in cecal digesta. Altogether, the results indicated that liquid feeding not only promoted growth performance but also improved intestinal health by enhancing gut barrier functions in weanling pigs.